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il-3 cytokine  (PeproTech)


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    Structured Review

    PeproTech il-3 cytokine
    Il 3 Cytokine, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il-3+cytokine/il+6+cytokine/bio_rxiv__2025__07__11__664317-168-64-65
    Average 90 stars, based on 1 article reviews
    il-3 cytokine - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cell Differentiation:

    Article Title: Alisertib inhibits acute myeloid leukemia cell growth by inhibiting STAT3 activation.
    Article Snippet: Aurora kinase A (AURKA) plays critical roles in the cell cycle.. Its oncogenic functions have been identified in various types of cancer.. However, its role in acute myeloid leukemia (AML) has not been extensively explored.

    Article Title: mTORC1-Dependent Regulation of the CCL24-CCR3 Axis Controls Granuloma Formation and Maintenance in Sarcoidosis
    Article Snippet: For c-Kit + and FcγRII/RIII + (CD16/32 + ) mast cell differentiation, bone marrow cells were cultured for 4 weeks on cell culture-treated dishes (one 6-well plate per mouse and 4 ml per well) in media: RPMI 1640 (Sigma), 10% low endotoxin FBS (Gibco), 2 mM L-glutamine (Sigma), 100 U/ml penicillin (Sigma), 100 μg/ml streptomycin (Sigma), 50 μg/ml β-mercaptothion (Gibco) supplemented with 5 ng/ml IL-3 (PeproTech) and 50ng/ml SCF(PeproTech).

    Article Title: Generation of two pairs of induced pluripotent stem cells from Angelman syndrome patients with class I 15q11.2-q13.1 deletions and their unaffected familial controls.
    Article Snippet: For reprogramming, PBMCs were thawed and cultured in StemProTM34 SFM medium (Gibco, 10639011) supplemented with IL-3, IL-6, FLT3 and SCF (PeproTech, 200–03, 200–06, 300–19, 300–07).

    Article Title: Understanding and reversing mammary tumor-driven reprogramming of myelopoiesis to reduce metastatic spread
    Article Snippet: 400 cells per well were plated in triplicate and cultured in StemPro 34 medium (ThermoFisher Scientific) supplemented with 100 ng/mL penicillin/streptomycin, 2mM L-Glutamine, SCF (25 ng mL −1 ), Flt3L (25 ng mL −1 ), IL-3 (10 ng mL −1 ), G-MCSF (10 ng mL −1 ), Epo (10 ng mL −1 ) and Tpo (25 ng mL −1 ) (Peprotech).

    Article Title: WNK1 mediates M-CSF-induced macropinocytosis to enforce macrophage lineage fidelity.
    Article Snippet: On day 5, cells were transferred to SITI media (Essential 6 medium containing 100 ng/mL SCF, 10 ng/mL IL-6, 30 ng/mL TPO (PeproTech, 300-18), and 30 ng/mL IL-3 (PeproTech, 200-03)), to complete their transition into myeloid progenitors.

    Article Title: Bioengineered immunocompetent preclinical trial-on-chip tool enables screening of CAR T cell therapy for leukaemia.
    Article Snippet: Since multiple types of cell were cultured within a single chip, cell culture media was chosen to mainly support the long-term maintenance of bone marrow immune cells and tissue structure with a mixture of SFEM-II (STEMCELL Technologies), EGM-2, RMPI1640 at a ratio of 1:2:1 and supplement with a cytokine cocktail (Thrombopoietin (TPO; catalogue number 300-18, Peprotech), IL-6 (catalogue number 200-06, Peprotech), IL-3 (catalogue number 200-03, Peprotech), FMS-like tyrosine kinase 3 ligand (Flt3-L; catalogue number 300-19, Peprotech) and stem cell factor (SCF; catalogue number 300-07, Peprotech), all at 12.5 ng ml−1) and recombinant human VEGF-165/VEGFA (catalogue number 230-00012- 10, RayBiotech) at 10 ng ml−1.

    Article Title: Exploiting FcRn engagement of an albumin-CpG oligonucleotide covalent conjugate for potent TLR9 immune induction
    Article Snippet: After electroporation, cells were resuspended in 300 μM DC medium (CellGenix, cat# 20805-0500) supplemented with 100 ng/ml Flt3-L, 0.2% P/S, 1 μM SR1, 50 μg/ml L-ascorbic acid (Sigma Aldrich), 20 ng/ml IL-3 (Peprotech, cat# 300-03), 100 ng/ml SCF, and 50 ng/ml TPO, and cells were differentiated to HSPC-pDCs till a total of 21 days of culture as previously described ( ).

    Article Title: Embryonic mast cells arise from the Cpa3-expressing precursors but not granulocyte-monocyte progenitors.
    Article Snippet: 1Department of Oncology at Xiangya Cancer Center and National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China 2Shanghai Institute of Immunology, Department of Gastroenterology and Center for Immune-Related Diseases at Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China 3Key Laboratory for Regenerative Medicine of Ministry of Education, Institute of Hematology, School of Medicine, Jinan University, Guangzhou 510632, China 4Laboratory of Experimental Hematology, Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China 5Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China 6Gustave Roussy Cancer Campus, Villejuif 94800, France 7Singapore Immunology Network, Agency for Science, Technology and Research, Singapore 138648, Singapore 8Shanghai Jiao Tong University School of Medicine-Yale Institute for Immune Metabolism (SYIIM), Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

    Cell Culture:

    Article Title: Alisertib inhibits acute myeloid leukemia cell growth by inhibiting STAT3 activation.
    Article Snippet: Aurora kinase A (AURKA) plays critical roles in the cell cycle.. Its oncogenic functions have been identified in various types of cancer.. However, its role in acute myeloid leukemia (AML) has not been extensively explored.

    Article Title: mTORC1-Dependent Regulation of the CCL24-CCR3 Axis Controls Granuloma Formation and Maintenance in Sarcoidosis
    Article Snippet: For c-Kit + and FcγRII/RIII + (CD16/32 + ) mast cell differentiation, bone marrow cells were cultured for 4 weeks on cell culture-treated dishes (one 6-well plate per mouse and 4 ml per well) in media: RPMI 1640 (Sigma), 10% low endotoxin FBS (Gibco), 2 mM L-glutamine (Sigma), 100 U/ml penicillin (Sigma), 100 μg/ml streptomycin (Sigma), 50 μg/ml β-mercaptothion (Gibco) supplemented with 5 ng/ml IL-3 (PeproTech) and 50ng/ml SCF(PeproTech).

    Article Title: Generation of two pairs of induced pluripotent stem cells from Angelman syndrome patients with class I 15q11.2-q13.1 deletions and their unaffected familial controls.
    Article Snippet: For reprogramming, PBMCs were thawed and cultured in StemProTM34 SFM medium (Gibco, 10639011) supplemented with IL-3, IL-6, FLT3 and SCF (PeproTech, 200–03, 200–06, 300–19, 300–07).

    Article Title: Understanding and reversing mammary tumor-driven reprogramming of myelopoiesis to reduce metastatic spread
    Article Snippet: 400 cells per well were plated in triplicate and cultured in StemPro 34 medium (ThermoFisher Scientific) supplemented with 100 ng/mL penicillin/streptomycin, 2mM L-Glutamine, SCF (25 ng mL −1 ), Flt3L (25 ng mL −1 ), IL-3 (10 ng mL −1 ), G-MCSF (10 ng mL −1 ), Epo (10 ng mL −1 ) and Tpo (25 ng mL −1 ) (Peprotech).

    Article Title: WNK1 mediates M-CSF-induced macropinocytosis to enforce macrophage lineage fidelity.
    Article Snippet: On day 5, cells were transferred to SITI media (Essential 6 medium containing 100 ng/mL SCF, 10 ng/mL IL-6, 30 ng/mL TPO (PeproTech, 300-18), and 30 ng/mL IL-3 (PeproTech, 200-03)), to complete their transition into myeloid progenitors.

    Article Title: Bioengineered immunocompetent preclinical trial-on-chip tool enables screening of CAR T cell therapy for leukaemia.
    Article Snippet: Since multiple types of cell were cultured within a single chip, cell culture media was chosen to mainly support the long-term maintenance of bone marrow immune cells and tissue structure with a mixture of SFEM-II (STEMCELL Technologies), EGM-2, RMPI1640 at a ratio of 1:2:1 and supplement with a cytokine cocktail (Thrombopoietin (TPO; catalogue number 300-18, Peprotech), IL-6 (catalogue number 200-06, Peprotech), IL-3 (catalogue number 200-03, Peprotech), FMS-like tyrosine kinase 3 ligand (Flt3-L; catalogue number 300-19, Peprotech) and stem cell factor (SCF; catalogue number 300-07, Peprotech), all at 12.5 ng ml−1) and recombinant human VEGF-165/VEGFA (catalogue number 230-00012- 10, RayBiotech) at 10 ng ml−1.

    Article Title: Exploiting FcRn engagement of an albumin-CpG oligonucleotide covalent conjugate for potent TLR9 immune induction
    Article Snippet: After electroporation, cells were resuspended in 300 μM DC medium (CellGenix, cat# 20805-0500) supplemented with 100 ng/ml Flt3-L, 0.2% P/S, 1 μM SR1, 50 μg/ml L-ascorbic acid (Sigma Aldrich), 20 ng/ml IL-3 (Peprotech, cat# 300-03), 100 ng/ml SCF, and 50 ng/ml TPO, and cells were differentiated to HSPC-pDCs till a total of 21 days of culture as previously described ( ).

    Article Title: Embryonic mast cells arise from the Cpa3-expressing precursors but not granulocyte-monocyte progenitors.
    Article Snippet: 1Department of Oncology at Xiangya Cancer Center and National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China 2Shanghai Institute of Immunology, Department of Gastroenterology and Center for Immune-Related Diseases at Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China 3Key Laboratory for Regenerative Medicine of Ministry of Education, Institute of Hematology, School of Medicine, Jinan University, Guangzhou 510632, China 4Laboratory of Experimental Hematology, Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China 5Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China 6Gustave Roussy Cancer Campus, Villejuif 94800, France 7Singapore Immunology Network, Agency for Science, Technology and Research, Singapore 138648, Singapore 8Shanghai Jiao Tong University School of Medicine-Yale Institute for Immune Metabolism (SYIIM), Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

    Recombinant:

    Article Title: Alisertib inhibits acute myeloid leukemia cell growth by inhibiting STAT3 activation.
    Article Snippet: Aurora kinase A (AURKA) plays critical roles in the cell cycle.. Its oncogenic functions have been identified in various types of cancer.. However, its role in acute myeloid leukemia (AML) has not been extensively explored.

    Article Title: mTORC1-Dependent Regulation of the CCL24-CCR3 Axis Controls Granuloma Formation and Maintenance in Sarcoidosis
    Article Snippet: For c-Kit + and FcγRII/RIII + (CD16/32 + ) mast cell differentiation, bone marrow cells were cultured for 4 weeks on cell culture-treated dishes (one 6-well plate per mouse and 4 ml per well) in media: RPMI 1640 (Sigma), 10% low endotoxin FBS (Gibco), 2 mM L-glutamine (Sigma), 100 U/ml penicillin (Sigma), 100 μg/ml streptomycin (Sigma), 50 μg/ml β-mercaptothion (Gibco) supplemented with 5 ng/ml IL-3 (PeproTech) and 50ng/ml SCF(PeproTech).

    Article Title: Generation of two pairs of induced pluripotent stem cells from Angelman syndrome patients with class I 15q11.2-q13.1 deletions and their unaffected familial controls.
    Article Snippet: For reprogramming, PBMCs were thawed and cultured in StemProTM34 SFM medium (Gibco, 10639011) supplemented with IL-3, IL-6, FLT3 and SCF (PeproTech, 200–03, 200–06, 300–19, 300–07).

    Article Title: Understanding and reversing mammary tumor-driven reprogramming of myelopoiesis to reduce metastatic spread
    Article Snippet: 400 cells per well were plated in triplicate and cultured in StemPro 34 medium (ThermoFisher Scientific) supplemented with 100 ng/mL penicillin/streptomycin, 2mM L-Glutamine, SCF (25 ng mL −1 ), Flt3L (25 ng mL −1 ), IL-3 (10 ng mL −1 ), G-MCSF (10 ng mL −1 ), Epo (10 ng mL −1 ) and Tpo (25 ng mL −1 ) (Peprotech).

    Article Title: WNK1 mediates M-CSF-induced macropinocytosis to enforce macrophage lineage fidelity.
    Article Snippet: On day 5, cells were transferred to SITI media (Essential 6 medium containing 100 ng/mL SCF, 10 ng/mL IL-6, 30 ng/mL TPO (PeproTech, 300-18), and 30 ng/mL IL-3 (PeproTech, 200-03)), to complete their transition into myeloid progenitors.

    Article Title: Bioengineered immunocompetent preclinical trial-on-chip tool enables screening of CAR T cell therapy for leukaemia.
    Article Snippet: Since multiple types of cell were cultured within a single chip, cell culture media was chosen to mainly support the long-term maintenance of bone marrow immune cells and tissue structure with a mixture of SFEM-II (STEMCELL Technologies), EGM-2, RMPI1640 at a ratio of 1:2:1 and supplement with a cytokine cocktail (Thrombopoietin (TPO; catalogue number 300-18, Peprotech), IL-6 (catalogue number 200-06, Peprotech), IL-3 (catalogue number 200-03, Peprotech), FMS-like tyrosine kinase 3 ligand (Flt3-L; catalogue number 300-19, Peprotech) and stem cell factor (SCF; catalogue number 300-07, Peprotech), all at 12.5 ng ml−1) and recombinant human VEGF-165/VEGFA (catalogue number 230-00012- 10, RayBiotech) at 10 ng ml−1.

    Article Title: Exploiting FcRn engagement of an albumin-CpG oligonucleotide covalent conjugate for potent TLR9 immune induction
    Article Snippet: After electroporation, cells were resuspended in 300 μM DC medium (CellGenix, cat# 20805-0500) supplemented with 100 ng/ml Flt3-L, 0.2% P/S, 1 μM SR1, 50 μg/ml L-ascorbic acid (Sigma Aldrich), 20 ng/ml IL-3 (Peprotech, cat# 300-03), 100 ng/ml SCF, and 50 ng/ml TPO, and cells were differentiated to HSPC-pDCs till a total of 21 days of culture as previously described ( ).

    Article Title: Embryonic mast cells arise from the Cpa3-expressing precursors but not granulocyte-monocyte progenitors.
    Article Snippet: 1Department of Oncology at Xiangya Cancer Center and National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China 2Shanghai Institute of Immunology, Department of Gastroenterology and Center for Immune-Related Diseases at Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China 3Key Laboratory for Regenerative Medicine of Ministry of Education, Institute of Hematology, School of Medicine, Jinan University, Guangzhou 510632, China 4Laboratory of Experimental Hematology, Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China 5Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China 6Gustave Roussy Cancer Campus, Villejuif 94800, France 7Singapore Immunology Network, Agency for Science, Technology and Research, Singapore 138648, Singapore 8Shanghai Jiao Tong University School of Medicine-Yale Institute for Immune Metabolism (SYIIM), Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

    In Vitro:

    Article Title: Alisertib inhibits acute myeloid leukemia cell growth by inhibiting STAT3 activation.
    Article Snippet: Aurora kinase A (AURKA) plays critical roles in the cell cycle.. Its oncogenic functions have been identified in various types of cancer.. However, its role in acute myeloid leukemia (AML) has not been extensively explored.

    Article Title: mTORC1-Dependent Regulation of the CCL24-CCR3 Axis Controls Granuloma Formation and Maintenance in Sarcoidosis
    Article Snippet: For c-Kit + and FcγRII/RIII + (CD16/32 + ) mast cell differentiation, bone marrow cells were cultured for 4 weeks on cell culture-treated dishes (one 6-well plate per mouse and 4 ml per well) in media: RPMI 1640 (Sigma), 10% low endotoxin FBS (Gibco), 2 mM L-glutamine (Sigma), 100 U/ml penicillin (Sigma), 100 μg/ml streptomycin (Sigma), 50 μg/ml β-mercaptothion (Gibco) supplemented with 5 ng/ml IL-3 (PeproTech) and 50ng/ml SCF(PeproTech).

    Article Title: Generation of two pairs of induced pluripotent stem cells from Angelman syndrome patients with class I 15q11.2-q13.1 deletions and their unaffected familial controls.
    Article Snippet: For reprogramming, PBMCs were thawed and cultured in StemProTM34 SFM medium (Gibco, 10639011) supplemented with IL-3, IL-6, FLT3 and SCF (PeproTech, 200–03, 200–06, 300–19, 300–07).

    Article Title: Understanding and reversing mammary tumor-driven reprogramming of myelopoiesis to reduce metastatic spread
    Article Snippet: 400 cells per well were plated in triplicate and cultured in StemPro 34 medium (ThermoFisher Scientific) supplemented with 100 ng/mL penicillin/streptomycin, 2mM L-Glutamine, SCF (25 ng mL −1 ), Flt3L (25 ng mL −1 ), IL-3 (10 ng mL −1 ), G-MCSF (10 ng mL −1 ), Epo (10 ng mL −1 ) and Tpo (25 ng mL −1 ) (Peprotech).

    Article Title: WNK1 mediates M-CSF-induced macropinocytosis to enforce macrophage lineage fidelity.
    Article Snippet: On day 5, cells were transferred to SITI media (Essential 6 medium containing 100 ng/mL SCF, 10 ng/mL IL-6, 30 ng/mL TPO (PeproTech, 300-18), and 30 ng/mL IL-3 (PeproTech, 200-03)), to complete their transition into myeloid progenitors.

    Article Title: Bioengineered immunocompetent preclinical trial-on-chip tool enables screening of CAR T cell therapy for leukaemia.
    Article Snippet: Since multiple types of cell were cultured within a single chip, cell culture media was chosen to mainly support the long-term maintenance of bone marrow immune cells and tissue structure with a mixture of SFEM-II (STEMCELL Technologies), EGM-2, RMPI1640 at a ratio of 1:2:1 and supplement with a cytokine cocktail (Thrombopoietin (TPO; catalogue number 300-18, Peprotech), IL-6 (catalogue number 200-06, Peprotech), IL-3 (catalogue number 200-03, Peprotech), FMS-like tyrosine kinase 3 ligand (Flt3-L; catalogue number 300-19, Peprotech) and stem cell factor (SCF; catalogue number 300-07, Peprotech), all at 12.5 ng ml−1) and recombinant human VEGF-165/VEGFA (catalogue number 230-00012- 10, RayBiotech) at 10 ng ml−1.

    Article Title: Exploiting FcRn engagement of an albumin-CpG oligonucleotide covalent conjugate for potent TLR9 immune induction
    Article Snippet: After electroporation, cells were resuspended in 300 μM DC medium (CellGenix, cat# 20805-0500) supplemented with 100 ng/ml Flt3-L, 0.2% P/S, 1 μM SR1, 50 μg/ml L-ascorbic acid (Sigma Aldrich), 20 ng/ml IL-3 (Peprotech, cat# 300-03), 100 ng/ml SCF, and 50 ng/ml TPO, and cells were differentiated to HSPC-pDCs till a total of 21 days of culture as previously described ( ).

    Article Title: Embryonic mast cells arise from the Cpa3-expressing precursors but not granulocyte-monocyte progenitors.
    Article Snippet: 1Department of Oncology at Xiangya Cancer Center and National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China 2Shanghai Institute of Immunology, Department of Gastroenterology and Center for Immune-Related Diseases at Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China 3Key Laboratory for Regenerative Medicine of Ministry of Education, Institute of Hematology, School of Medicine, Jinan University, Guangzhou 510632, China 4Laboratory of Experimental Hematology, Fifth Medical Center of Chinese PLA General Hospital, Beijing 100039, China 5Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China 6Gustave Roussy Cancer Campus, Villejuif 94800, France 7Singapore Immunology Network, Agency for Science, Technology and Research, Singapore 138648, Singapore 8Shanghai Jiao Tong University School of Medicine-Yale Institute for Immune Metabolism (SYIIM), Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China



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